Binding of radiographic contrast media to serum proteins demonstrated by immunoelectrophoresis and radioautography.

BINDING OF RADIOGRAPHIC CONTRAST MEDIA TO SERUM PROTEINS A clinical and experimental investigation of their adverse effects through influence on active steroid hormone levels. by Staffan Wirell, M.D., Department of Diagnostic Radiology, University Hospital of Umeå, S-901 85 Umeå, Sweden. Summary of thesis based on the following papers: I Wirell S.: Adverse reactions at intravenous cholegraphy with ioglycamide. Correlation with the level of circulating sex hormones. Accepted for publication in Acta Radiol. Diagn. (1982) II Wirell S.: Effects of steroid hormones on the binding of ioglycamide (Bilivistan) to human blood serum. Acta Radiol. Diagn. 19 (1978), 289. III Wirell S.: Release of protein bound progesterone by ioglycamide in human serum. Accepted for publication in Acta Radiol. Diagn. (1982). IV Wirell S., Södergård R. and Selstam G.: Ioglycamide induced release of testosterone and estradiol from protein bound stores. Accepted for publication in Acta Radiol. Diagn. (1982). V Wirell S.: Binding of metrizamide to human serum proteins. Accepted for publication in Acta Radiol. Diagn. (1982). In a clinical study, Paper I, the highest incidence of adverse reactions at intra venous cholegraphy was recorded in young adults; especially in the luteal phase of the menstrual cycle. In Papers II, III and IV, the interplay between the protein binding of a bileexcreted contrast compound, ioglycamide, and steroid sex hormones was studied. Altered binding characteristics of the steroid hormone-protein binding indic­ ated the possibility of a release of steroid hormones in the presence of ioglyc­ amide. The significance of this finding was discussed in terms of clinical relevance. In Paper V, the binding of a non-ionic contrast compound to human serum proteins was studied with two different techniques. No, or only little, binding of metriz amide to human serum proteins was demonstrated.